Der Lab Plasmids Deposited at Addgene |
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Plasmid |
Encoded Protein |
Species |
Description |
Reference(PubMed ID) |
| pBabe N-Ras 61K | N-Ras(Q61K) | Human | GTPase-deficient, constitutively activated | 11463831 |
| pBabe K-Ras 12V | K-Ras(G12V) | Human | GTPase-deficient, constitutively activated | 11463831 |
| pBabe H-Ras 12V | H-Ras(G12V) | Human | GTPase-deficient, constitutively activated | 11463831 |
| pBabe puro H-Ras 12V (35S) | H-Ras(G12V/A35S) | Human | Effector domain mutant, retains the ability to activate Raf, but impaired activation of PI3K and RalGEF | 15352030 |
| pBabe puro H-Ras 12V (37G) | H-Ras(G12V/E37G) | Human | Effector domain mutant, retains the ability to activate RalGEF, but impaired activation of PI3K and Raf. | 15352030 |
| pBabe puro H-Ras 12V (40C) | H-Ras(G12V/Y40C) | Human | Effector domain mutant, retains the ability to activate PI3K, but impaired activation of Raf and RalGEF. | 15352030 |
| pBabe hygro myc p110-CAAX | p110-CAAX | Bovine | p110a chimeric protein terminating with C‐terminal prenylation signal sequence of human K‐Ras4B; contains N-terminal Myc epitope tag | 11463831 |
| pBabe HAII RLF CAAX | RGL2/RLF-CAAX | Mouse | Chimeric protein terminating with C‐terminal 18 amino acid plasma membrane targeting sequence of K‐Ras4B; Rlf lacks the C‐terminal 247 residues that contain the Ras association domain. |
11463831 |
| pBabe puro C-Raf 22W | DN-Raf-1 | Human | N-terminal deletion of residues 1-305, including the Ras-binding domain and autoinhibitor sequences, creating a constitutively activated, cytosolic and transforming mutant | 2710120 |
| Raf-1 GST RBD 1-149 | GST-Raf-RBD | Human | pGEX-2T bacterial expression vector encoding GST followed by N-terminal residues 2-149 of human Raf-1. Contains the Ras-binding domain that binds preferentially to activated Ras-GTP. Used for determination of Ras-GTP formation in mammalian cell lysates | 7730360 |
| pBabe puro pI3K p110 CAAX | p110-CAAX | Bovine | p110a chimeric protein terminating with C‐terminal prenylation signal sequence of human K‐Ras4B; contains N-terminal Myc epitope tag |
|
| pBabe puro H-Ras 61L | H-Ras(Q61L) | Human | GTPase-deficient, constitutively activated | 11305105 |
| pBabe B-Raf V600E | B-Raf(V600E) | Human | Mutationally-activated by most common tumor-associated mutation | 17699719 |
| pBabe puro RalA (long) wt | RalA | Human | Wild type | 15950903 |
| pBabe puro RalA S31N | RalA | Human | Dominant-negative mutant, analogous to Ras(S17N), forms an inactive complex with RalGEFs | 15950903 |
| pBabe puro RalA Q75L | RalA(Q75L) | Human | GTPase-deficient, constitutively activated; analogous to Ras(Q61L) | 15950903 |
| pBabe puro RalB wt | RalB | Human | Wild type | 15950903 |
| pBabe puro RalB Q72L | RalB(Q72L) | Human | GTPase-deficient, constitutively activated; analogous to Ras(Q61L) | 15950903 |
| pBabe puro RalB S28N | RalB(S28N) | Human | Dominant-negative mutant, analogous to Ras(S17N); forms an inactive complex with RalGEFs | 15950903 |
| pBabe puro RalA (short) wt | RalA | Human | Wild type | 15950903 |
| GFP-RhoA | RhoA | Human | GFP-tagged at N-terminus | 18614539 |
| GFP-RhoB | RhoB | Human | GFP-tagged at N-terminus | 18614539 |
| GFP-RhoC | RhoC | Human | GFP-tagged at N-terminus | 18614539 |
| GFP-Rnd1 | Rnd1 | Human | GFP-tagged at N-terminus | 18614539 |
| GFP-Rnd2 | Rnd2 | Human | GFP-tagged at N-terminus | 18614539 |
| GFP-Rnd3 | Rnd3 | Human | GFP-tagged at N-terminus | 18614539 |
| GFP-RhoH | RhoH | Human | GFP-tagged at N-terminus | 18614539 |
| GFP-TCL | TCL | Human | GFP-tagged at N-terminus | 18614539 |
| GFP-TC10 | TC10 | Human | GFP-tagged at N-terminus | 18614539 |
| GFP-Rif | Rif | Human | GFP-tagged at N-terminus | 18614539 |
| GFP-hRhoD | RhoD | Human | GFP-tagged at N-terminus | 18614539 |
| GFP-mRhoD | RhoD | Mouse | GFP-tagged at N-terminus | 18614539 |
| GFP-pCdc42 | Cdc42 (placental) | Human | GFP-tagged at N-terminus | 18614539 |
| GFP-bCdc42 | Cdc42 (brain) | Human | GFP-tagged at N-terminus | 18614539 |
