To Upload your images and files into UNC database

1) UNC Microarray Database (UMD)

• Go to UNC database web site: https://genome.unc.edu/
• Fill out the "Registration Form" online and submit to set up your user name and get your password. Your user name and password will be automatically emailed to you.
• After login to the UMD (https://genome.unc.edu/), under “Enter Data”; find and click "upload file" to load your file from your computer to the UMD.

2) How to match your array data to the correct design file?

• Go back to "Enter Data" page; click "Experiment and Results".
• Click "Enter a New Experiment into the Database"; choose the correct organism such as Human or mouse.
• Choose correct scanner: Example: choose Agilent if your array was scanned by Agilent Scanner.
• Select correct Print Name: Example: You use Agilent human 4x44K array with bar code 252665212345, the 26652 is the design code of this array. You should select a printing name with same design # at the end printing name (BARCODE26652) as your UNC MD print name.
• Fill same bar code for all 4 arrays for each of 4x44K slide
• Use your microarray image file (.tif) for Agilent Scan File_H; create a dummy.tif using notepad for Agilent Scan File_L.
• Use same image.tif and dummy.tif for all your 4 arrays.
• You have to load each of your array files one by one. You also need to wait a while to allow the database time to transfer your files. You will get an email to notice your data transferred successfully! (Call Yan Shi 919-972-7487 if you need help for file upload).

3) How to see your array data

After all four files are loaded in database and match your array design files, you can view their details and check their quality. Select "Advanced Results Search" in "Search" section. Choose based on experiment name and array categories and subcategories, press display data button. You are now at the "Search Results" page. Your arrays are listed and you can choose to see your raw data, image of array spots, and many ways to plot raw data to analyze their quality.