 GC -80C Freezer location |
 Follow the Instruction |
 Shelf and Empty Plates |
|
|
1. Concentration and Quality Check:
- RNA concentration measurement by NanoDrop: Print, fill the request form and submit form with samples
- DNA concentration measurement by Qubit: Print, fill the request form and submit form with samples
- RNA quality check by Bioanalyzer: Print, fill the request form and submit form with samples
- DNA fragments check by Bioanalyzer: Print, fill the request form and submit form with samples
2. Microarrays:
- Our system and equipment are set up for all Agilent high density microarrays.
- All samples for microarray analysis should be in 0.2 ml individual flat-top PCR tubes.
- Call Genomics Core for schedule pick up
- Gene Expression Array:
- The gene expression microarrays from Agilent are printed as 4 or 8 identical arrays on 1 slide. Plan your experiments with 4 or 8 samples to avoid wasting arrays.
- Labeling is amplification with Cy3- and Cy5-labeled dNTP in separate reactions to produce differentially labeled sample and reference c-DNAs. Both are hybridized to the same microarray.
- Print and complete the request form and submit form with samples and microarrays.
- microRNA Array:
- The microRNA microarrays from Agilent are printed as 8 identical microarrays on 1 slide. Plan your experiments with 8 samples to avoid wasting arrays.
- Dephosphorylate and label method with Cy-dye (Cy3-pCp) for total RNA or small RNA samples.
- The amount of initial RNA is strongly depended on the samples sources and RNA type.
- Print and complete the request form and submit form with samples and microarrays.
- CGH array:
- 2 color direct label method with Cy-Dye (Cy3 and Cy5) for sample genomic DNA vs reference genomic DNA; both labeled sample DNA and reference DNA hybridize on a same Agilent high density microarrays;
- 3 µg of both genomic DNA sample and genomic DNA reference are requires for initial process.
- If use 4x microarrays, plan your experiments with 4 samples to avoid wasting arrays
- Print and complete the request form and submit form with samples and microarrays
3. mRNA library Preperation:
- preparing mRNA library for high throughput sequence analysis;
- For each sample, 1-2 µg total RNA (20-30 µl) is required for initial process
- Print and complete the request form and submit form with samples
|