1. Concentration and Quality Check:

• RNA concentration measurement by NanoDrop: Print, fill the request form and submit form with samples
• DNA concentration measurement by Qubit: Print, fill the request form and submit form with samples
• RNA quality check by Bioanalyzer: Print, fill the request form and submit form with samples
• DNA fragments check by Bioanalyzer: Print, fill the request form and submit form with samples

2. Microarrays:

• Our system and equipment are set up for all Agilent high density microarrays.
• All samples for microarray analysis should be in 0.2 ml individual flat-top PCR tubes.
• Call Genomics Core for schedule pick up


• Gene Expression Array:
1. The gene expression microarrays from Agilent are printed as 4 or 8 identical arrays on 1 slide. Plan your experiments with 4 or 8 samples to avoid wasting arrays.
2. Labeling is amplification with Cy3- and Cy5-labeled dNTP in separate reactions to produce differentially labeled sample and reference c-DNAs. Both are hybridized to the same microarray.
3. Print and complete the request form and submit form with samples and microarrays.


• microRNA Array:
1. The microRNA microarrays from Agilent are printed as 8 identical microarrays on 1 slide. Plan your experiments with 8 samples to avoid wasting arrays.
2. Dephosphorylate and label method with Cy-dye (Cy3-pCp) for total RNA or small RNA samples.
3. The amount of initial RNA is strongly depended on the samples sources and RNA type.
4. Print and complete the request form and submit form with samples and microarrays.


• CGH array:
1. 2 color direct label method with Cy-Dye (Cy3 and Cy5) for sample genomic DNA vs reference genomic DNA; both labeled sample DNA and reference DNA hybridize on a same Agilent high density microarrays;
2. 3 µg of both genomic DNA sample and genomic DNA reference are requires for initial process.
3. If use 4x microarrays, plan your experiments with 4 samples to avoid wasting arrays
4. Print and complete the request form and submit form with samples and microarrays

3. mRNA library Preperation:

• preparing mRNA library for high throughput sequence analysis;
• For each sample, 1-2 µg total RNA (20-30 µl) is required for initial process
• Print and complete the request form and submit form with samples