The Nucleic Acids Core Facility (NACF) provides investigators
with low-cost, high-quality oligonucleotides for use in a
wide range of
applications including DNA sequencing, PCR, cDNA/RNA synthesis, hybridization,
in vitro mutagenesis and anti-sense inhibition of gene expression.
Oligonucleotides are available at five synthesis scales ranging from
40 nmoles to 10 umoles.
A wide range of custom synthesis scales are available, including RNA
oligomers, phosphorthioate oligomers, degenerate oligos and labeling
fluorescein and many other compounds. All oligos are tested by gel
electrophoresis to verify successful synthesis.
Oligonucleotides are provided deprotected and dried. Most users find
that no purification is needed before use in PCR and manual DNA sequencing.
The NACF provides custom purification services for users who require
more highly purified synthetic nucleic acids.
The NACF also maintains a freezer stocked with PCR-related enzymes and
reagents from Perkin-Elmer. Customers using the freezer receive a discounted
price and pay no shipping charges.
| Lineberger Comprehensive Cancer Center Nucleic Acids Core Facility
Announces: New Pricing Beginning April 1st, 2008
Oligos: now $0.19/base (40nmole, desalted, for cancer center members)
Also, NACF has teamed up with Sigma-Genosys to provide our users
(both LCCC members and non-members) with high quality and low price
specialty oligos including Dual-labeled probes, RNA, siRNA and
Dual-Labeled probes: 1OD for $89.10 or 3 OD for $163.9 (price
includes dual labels, HPLC purification and base charges, applying
to 15-40 bases in length)
siRNA: 2 OD for $115.5 or 5 OD for $192.5 (desalted)
RNA: $1.93 per base (50 nmole)
Room 11-116 of the Lineberger Cancer Center Building.
As of December 17, 2012, Nucleic Acids Core Facility relocates its office to Room 31-321 (next to Tissue Culture Facility) of the LCCC building.
Hours of Operation
8:45 a.m. to 2:45 p.m.
Monday through Friday
Steve Oglesbee: (919) 966-4324
Sharon Daniel: (919) 966-4324
Prices (per base)
||$0.19 (reduced price)
||$0.38 (reduced price)
||$0.66 (reduced price)
||$1.32 (reduced price)
||$1.20 (reduced price)
||$2.40 (reduced price)
||$12.00 + $32/oligo
||$15.00 + $32/oligo