Ordering information

Oligos can be ordered via the internet.

Please follow this link to place your order now.

Oligo orders can still be dropped off at the facility. Turnaround time for 40 nmole orders averages 2 days. We will email you when your oligo is ready.

Please use a separate order form for each oligonucleotide. If you need a form follow the link below and print a copy.

Click here to dowload the Request Form in MS-Word format

Please fill out the form carefully according to the following instructions:

Name: Requestor's name.
Building/Room#: Location of the requestor's laboratory. If this is your first order, please provide us with your department name and campus box address.
Billing PI (Full Name): Name of the principle investigator to whom the bill should be sent.
Billing Account Number: 6-digit number of the billing account, i.e.: 6-54321
Email address and phone # : We will email you or call this number when the oligo is ready.
Date: Date placing the order.
Length: ___mer Number of bases in the requested oligo.
Sequence (In Triplets 5' -->3') Please write clearly using A, g, C, T or the IUB degeneracy codes shown at the bottom of the order form. There is no charge for degeneracies in positions other than the 3' end.
List Modifications: If you have included any non-ATgC nucleotides in your sequence, or if you are requesting end labeling with biotin, fluorescein, phosphate, etc, indicate them here and in your sequence. Click here to see a list of modifications and their prices.
Optional Purification: If you want us to carry out "Trityl On" purification ($15 additional charge per oligo) or SepPak desalting (free), circle as appropriate. "Trityl On" is unavailable for 40 nM scale oligonucleotides more than 50 base pairs.
Designation: Your name for the oligo. Please make this 8 characters or less.
Scale of Synthesis: Scale refers to the theoretical molar yield of the synthesis, although in most cases the actual yield of a synthesis is significantly lower. The approximate molar yields for each of the available synthesis scales are as follows:

Synthesis

The 40 nmole crude oligos are suitable for most applications including sequencing, PCR and cloning. A 25-mer synthesized at this scale will provide about 250 µg of crude DNA.

Final products:
For desalted and purified oligos: yield of the oligo is measured in OD unit, µg and nmol. (check the bottom of your request form).
For crude oligos -- check the concentration by spectrophotometer.