 |
 |
|
 |
||
 |
 |
|
 |
|
|
Flow Cytometry Facility
Room: 616/618 Faculty Laboratory Office BuildingDirector(s): Larry W. Arnold, Ph.D., Faculty Director
Hours: M-F 8:00 a.m.- 5:00 p.m. (Other times available by special arrangement- please call 966-1530)
Phone: (919) 966-1530
Fax: (919) 962-8103
Email: lwarma@med.unc.edu
Services:
The Flow Cytometry Facility provides state-of-the-art analytic and sorting technologies and services to Cancer Center members.
The Facility supports analyses for various applications and different cell types:
cell surface immunofluorescence using one-four color analysis
cytoplasmic or nuclear antigen immunofluorescence
univariate cell cycle and ploidy analysis using propidium iodide labeling or bivariate S-phase analysis with BrdU
Apoptosis monitoring using single or two color methods, including TdT fragment labeling
calcium mobilization
The Facility performs sorting of cell populations:
to purify cells based on cell membrane molecules or other labels
to isolate, enrich, or clone mutants and cells expressing transfected gene products
The Facility offers data analysis using a variety of flow cytometric data analysis software packages.
Facility staff can help with generation of publication quality graphics.
The Facility offers training or consultation in the following areas:
instrumentation theory, instrument operation by investigators and their staff, and data acquisition and analysis. Analysis software is available to investigators for use on their own lab computers or free of charge on the Facility computer.
study design, sample preparation and methods development
Staff:
The Faculty Director is Dr. Larry Arnold, Research Professor of Microbiology and Immunology. Nancy Martin is the research technician.
Location:
The Flow Cytometry Facility is located in Room 616 and 618 of the Faculty Laboratory Office Building
For More Information:
For more information, visit the Flow Cytometry Facility or telephone 966-1530 Web site:
http://flowcytometry.med.unc.edu
