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Last Updated: 9/8/2009
| Ivan Rusyn, M.D., Ph.D.
Associate Professor |  |
Research Interests
Our laboratory applies molecular, biochemical, genetic and genomics approaches to understanding the mechanisms of environmental agent-related organ injury and carcinogenesis. Specifically, we are interested in the genetic determinants of the susceptibility to toxicant-induced liver injury, nuclear receptor-mediated pathways in chemical carcinogenesis, oxidative DNA damage and repair, and the role that alcohol and diet play in cancer. Through a combination of in vivo animal studies and experiments that utilize cellular and molecular models, we aim to better understand why certain chemicals cause cancer or organ-specific toxicity and whether humans in general, or any susceptible sub-population in particular, are at risk from similar exposures.
FUNDED PROJECTS:
R01-ES015241(12/07–11/12)
National Institute of Environmental Health Sciences
"Bioengineering Partnership to Improve Chemical Hazard Testing Paradigms"
In this project we apply an integrative systems biology approach to: (1) Develop a 3D microscale mouse liver tissue bioreactor that can be used for high-throughput screening of chemicals; (2) Build, test and validate a quantitative structure-toxicity relationship model that takes into account genetic diversity among individuals; and (3) Validate a fiscally sensible in vivo and in vitro toxicity screening paradigm for a class of allylbenzene derivatives by producing knowledge anchored on the genetic variability present within the population.
STAR-RD833825 (4/08-3/12)
US Environmental Protection Agency
"Carolina Center for Computational Toxicology"
The Center will advance the field of computational toxicology through the development of new methods and computational tools, as well as through inter-disciplinary collaborative efforts within UNC and with other environmental health science researchers.
R01-AA16258 (9/05-7/10)
National Institute of Alcohol Abuse and Alcoholism
"Metabolomic and Toxicogenetic Study of Ethanol Toxicity"
The aim of this proposal is to define a “liver toxicity susceptibility state” in mouse liver in response to alcohol drinking by combining knowledge of toxicology, metabolomics, gene expression profiling and mouse genetics.
STAR-RD832720 (9/05-8/10)
US Environmental Protection Agency
"Carolina Environmental Bioinformatics Center: Project #3"
We work closely with the EPA’s National Center for Computational Toxicology, located nearby in Research Triangle Park, NC on the interdisciplinary project which brings together experts in the fields of biostatistics, computer science, environmental sciences and engineering, genetics, medicinal chemistry, and chem-informatics. The goals of the Center are to develop innovative methods and tools for improving linkages in the source-to-outcome paradigm, hazard identification and quantitative risk assessment; to provide an efficient and rapid resource for interaction between scientists and regulators; and to translate and apply the scientific discoveries of the Center into web-based resources for policy makers and the public.
P42-ES005948 (4/06-3/10)
National Institute of Environmental Health Sciences
"Environmental Exposure and Effect of Hazardous Chemicals: Project #2"
This project explores the toxicogenetic approach to studies of trichloroethylene-induced liver and kidney toxicity and carcinogenesis. We aim to determine what genetic variants correlate with susceptibility or resistance to liver and kidney disease due to this ubiquitous environmental contaminant, thus potentially helping to identify susceptible individuals.
Recent Accomplishments and Honors
2008 Achievement Award, Society of Toxicology
2002-2005 Transition to Independent Position Award, NIEHS
2000-2002 Individual Postdoctoral National Research Service Award, NIEHS
2000-2001 Leon & Bertha Golberg Memorial Postdoctoral Fellowship, UNC-Chapel Hill
2000 Visiting Professor of Surgery, Yamanashi Medical University, Japan
2000 Young Investigator Award, Soc. for Free Radical Research International
2000 AACR - Bristol Myers Squibb Oncology Young Investigator Scholar Award
2000 Carl C. Smith Mechanisms Specialty Section Award, Society of Toxicology
1998/99/2001 Young Investigator Award, Oxygen Society
1995-1996 Research Fellowship, German Academic Exchange Service (DAAD)
1994 First Class Honors Diploma, Ukrainian State Medical University, Kiev, Ukraine
Training
Ukrainian State Medical University, M.D.(with Honors), 1994
University of Dusseldorf, Germany, Postdoctoral, 1995-1996
University of North Carolina at Chapel Hill, Ph.D., 2000
University of North Carolina at Chapel Hill, Postdoctoral, 2000-2001
Massachusetts Institute of Technology, Postdoctoral, 2001-2002
Publications
Most Recent Publications (from 80+ total):
1. Harrill, A.H., Watkins, P.B., Su, S., Ross, P.K., Harbourt, D.E., Stylianou, I.M., Boorman, G.A., Russo, M.W., Sackler, R.S., Harris, S.C., Smith, P.C., Tennant, R., Bogue, M., Paigen, K., Harris, C., Contractor, T., Wiltshire, T., Rusyn, I., and Threadgill, D.W. Mouse population-guided resequencing reveals that variants in CD44 contribute to acetaminophen-induced liver injury in humans. Genome Res 19:1507-1515, 2009.
2. Gatti, D.M., Harrill, A.H., Wright, F.A., Threadgill, D.W., and Rusyn, I. Replication and narrowing of gene expression quantitative trait loci using inbred mice. Mamm Genome 20:437-446, 2009.
3. Kim, S., Collins, L.B., Boysen, G., Swenberg, J.A., Gold, A., Ball, L.M., Bradford, B.U., and Rusyn, I. Liquid chromatography electrospray ionization tandem mass spectrometry analysis method for simultaneous detection of trichloroacetic acid, dichloroacetic acid, S-(1,2-dichlorovinyl)glutathione and S-(1,2-dichlorovinyl)-L-cysteine. Toxicology 262:230-238, 2009.
4. Zhu, H., Ye, L., Richard, A., Golbraikh, A., Rusyn, I., and Tropsha, A. A novel two-step hierarchical quantitative structure activity relationship modeling workflow for predicting acute toxicity of chemicals in rodents. Envr Health Persp 117:1257-1264, 2009.
5. Harrill, A.H., Ross, P.K., Threadgill, D.W., and Rusyn, I. Population-based discovery of toxicogenomics biomarkers for hepatotoxicity using a laboratory strain diversity panel. Toxicol Sci 110:235-243, 2009.
6. Pogribny, I.P., Tryndyak, V.P., Bagnyukova, T.V., Melnyk, S., Montgomery, B., Ross, S.A., Latendresse, J.R., Rusyn, I., and Beland, F.A. Hepatic epigenetic phenotype predetermines individual susceptibility to hepatic steatosis in mice fed a lipogenic methyl-deficient diet. J Hepatol 51: 176-186, 2009.
7. Kim, S., Kim, D., Pollack, G.M., Collins, L.B., and Rusyn, I. Pharmacokinetic analysis of trichloroethylene metabolism in male B6C3F1 mice: Formation and disposition of trichloroacetic acid, dichloroacetic acid, S-(1,2-dichlorovinyl)glutathione and S-(1,2-dichlorovinyl)-L-cysteine. Toxicol Appl Pharmacol 238: 90-99, 2009.
8. Ross, P.K., Woods, C.G., Bradford, B.U., Kosyk, O., Gatti, D.M., Cunningham, M.L., and Rusyn, I. Time-course comparison of xenobiotic activators of CAR and PPARalpha in mouse liver. Toxicol Appl Pharmacol 235:199-207, 2009.
9. Gatti, D.M., Sypa, M., Rusyn, I., Wright, F.A., and Barry, W.T. SAFEGUI: Resampling-based tests of categorical significance in gene expression data made easy. Bioinformatics 25:541–542, 2009.
10. Gatti, D.M., Shabalin, A.A., Lam, T.C., Wright, F.A., Rusyn, I., and Nobel, A.B. FastMap: Fast eQTL mapping in homozygous populations. Bioinformatics 25: 482-489, 2009.
E-mail: iir@unc.edu
Telephone: 919-843-2596
FAX: 919-843-2596
Address: 0031 Michael Hooker Research Center Chapel Hill, NC 27599-7431
